54827-17-7
- Product Name：Tetramethylbenzidine
- Molecular Formula：C16H20N2
- Purity：99%
- Molecular Weight：240.34
Product Details:
CasNo： 54827-17-7
Molecular Formula： C16H20N2
Appearance： White or pale yellow solid
Delivery Time： 2 weeks after order
Throughput： 100kg/Month
Purity： 99%
Synonyms: (1'-biphenyl)-4,4-diamine,3,3',5,5'-tetramethyl-
Density: 1.1±0.1 g/cm3
Boiling point: 368.6±37.0 °C at 760 mmHg
Flash point: 210.8±26.0 °C
Vapor pressure: 0.0±0.8 mmHg at 25°C
Refractive index: 1.618
Solubility: Slightly soluble. <0.1 g/100 mL at 20°C.
Pka: 4.49±0.10
Water Solubility: Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive: Light Sensitive
Stability: Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
Description:
Tetramethylbenzidine is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. Tetramethylbenzidine is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.
Uses:
Tetramethylbenzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.
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